Horje
opencv(4.4.0) c:\users\appveyor\appdata\local\temp\1\pip-req-build-71670poj\opencv\modules\imgproc\src\color.cpp:182: error: (-215:assertion failed) !_src.empty() in function Code Example
cv2.error: OpenCV(4.5.3) C:\Users\runneradmin\AppData\Local\Temp\pip-req-build-q3d_8t8e\opencv\modules\imgproc\src\color.cpp:182: error: (-215:Assertion failed) !_src.empty() in function 'cv::cvtColor' 
Try Giving Full Path. 
This seems to be the path issue in windows. 
I changed it to a full path like this and it worked.

filename = "D:\Sandbox\Github\opencv-project\Resources\Photos\cats.jpg"
opencv(4.4.0) c:\users\appveyor\appdata\local\temp\1\pip-req-build-71670poj\opencv\modules\imgproc\src\color.cpp:182: error: (-215:assertion failed) !_src.empty() in function 
This error happened because the image didn't load properly . So you have problem with the previous line cv2.imread my suggestion is :

check if the images exist in the path you give

check the count variable if he have valid number



Whatever

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Horje